Human bone marrow-derived mesenchymal stem cells (hBMSCs) and their by-product enhanced inexperienced fluorescent protein (eGFP)-hBMSCs had been employed to judge an modern hybrid scaffold composed of granular hydroxylapatite and collagen hemostat (Coll/HA). The mobile morphology/cytoskeleton group and cell viability had been investigated by immunohistochemistry (IHC) and AlamarBlue metabolic assay, respectively. The expression of osteopontin and osteocalcin proteins was analyzed by IHC and ELISA, whereas osteogenic genes had been investigated by quantitative PCR (Q-PCR). Cell morphology of eGFP-hBMSCs was indistinguishable from that of parental hBMSCs. The cytoskeleton structure of hBMSCs grown on the scaffold gave the impression to be nicely organized, whereas its integrity remained uninfluenced by the scaffold through the time course.
Metabolic exercise measured in hBMSCs grown on a biomaterial was elevated through the experiments, as much as day 21 (p < 0.05). The biomaterial induced the matrix mineralization in hBMSCs. The scaffold favored the expression of osteogenic proteins, akin to osteocalcin and osteopontin. In hBMSC cultures, the scaffold induced up-regulation in particular genes which can be concerned in ossification course of (BMP2/3, SPP1, SMAD3, and SP7), whereas they confirmed an up-regulation of MMP9 and MMP10, which play a central function through the skeletal growth.
hBMSCs had been induced to chondrogenic differentiation by means of up-regulation of COL2A1 gene. Our experiments counsel that the modern scaffold examined herein offers a very good microenvironment for hBMSC adhesion, viability, and osteoinduction. hBMSCs are a wonderful in vitro mobile mannequin to assay scaffolds, which could be employed for bone restore and bone tissue engineering.
Topographic Steerage in Soften-Electrowritten Tubular Scaffolds Enhances Engineered Kidney Tubule Efficiency
Thus far, tubular tissue engineering depends on giant, non-porous tubular scaffolds (Ø > 2 mm) for mechanical self-support, or smaller (Ø 150-500 μm) tubes inside bulk hydrogels for learning renal transport phenomena. To advance the engineering of kidney tubules for future implantation, constructs ought to be each self-supportive and but small-sized and extremely porous. Right here, we hypothesize that the fabrication of small-sized porous tubular scaffolds with a extremely organized fibrous microstructure by the use of melt-electrowriting (MEW) permits the event of self-supported kidney proximal tubules with enhanced properties.
A custom-built melt-electrowriting (MEW) machine was used to manufacture tubular fibrous scaffolds with small diameter sizes (Ø = 0.5, 1, Three mm) and well-defined, porous microarchitectures (rhombus, sq., and random). Human umbilical vein endothelial cells (HUVEC) and human conditionally immortalized proximal tubular epithelial cells (ciPTEC) had been seeded into the tubular scaffolds and examined for monolayer formation, integrity, and group, in addition to for extracellular matrix (ECM) manufacturing and renal transport performance.
Tubular fibrous scaffolds had been efficiently manufactured by high-quality management of MEW instrument parameters.
A minimal inside diameter of 1 mm and pore sizes of 0.2 mm had been achieved and used for subsequent cell experiments. Whereas HUVEC had been unable to bridge the pores, ciPTEC shaped tight monolayers in all scaffold microarchitectures examined. Effectively-defined rhombus-shaped pores outperformed and facilitated unidirectional cell orientation, elevated collagen kind IV deposition, and expression of the renal transporters and differentiation markers natural cation transporter 2 (OCT2) and P-glycoprotein (P-gp).
Right here, we current smaller diameter engineered kidney tubules with microgeometry-directed cell performance. Because of the well-organized tubular fiber scaffold microstructure, the tubes are mechanically self-supported, and the self-produced ECM constitutes the one barrier between the inside and outer compartment, facilitating speedy and energetic solute transport.
Exosomes as a promising therapeutic technique for peripheral nerve harm
Peripheral nerve harm has a excessive incidence and sometimes results in extreme losses of sensory and motor features within the limb. Autologous nerve grafts are broadly accepted because the gold commonplace for peripheral nerve restore, however the presence of inherent drawbacks dramatically reduces their usability. Quite a few tissue engineering nerve grafts are developed as options of autologous nerve grafts, and quite a lot of cells and neurotrophic elements had been launched into these grafts for enchancment.
Nonetheless, they’re nonetheless troublesome to acquire passable scientific outcomes. Peripheral nerve regeneration following harm stays a major problem for researchers and clinicians. Exosomes are extracellular membranous nanovesicles which can be secreted by most cells. As the important thing gamers of intercellular communication, exosomes play a elementary function within the physiological and pathological processes of the nervous system. Accumulating proof has recommended that exosomes can exert neurotherapeutic results through mediating axonal regrowth, Schwann cell activation, vascular regeneration, and inflammatory regulation.
 Anti-Tissue Factor Rabbit Monoclonal Antibody |
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| M00342 | BosterBio | 100ug/vial | EUR 397 |
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Description: Rabbit Monoclonal Tissue Factor Antibody. Validated in IF, IHC-P, ICC, WB and tested in Human, Mouse, Rat. |
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 Anti-Connective Tissue Growth Factor antibody |
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| STJ73587 | St John's Laboratory | 100 µg | EUR 359 |
 Anti-Tissue Factor Pathway Inhibitor antibody |
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| STJ70019 | St John's Laboratory | 100 µg | EUR 359 |
 Tissue Factor antibody |
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| 20R-1401 | Fitzgerald | 10 mg | EUR 303 |
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Description: Sheep polyclonal Tissue Factor antibody |
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 Tissue Factor Antibody |
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| 49112-100ul | SAB | 100ul | EUR 333 |
Tissue Factor Antibody |
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| 49112-50ul | SAB | 50ul | EUR 239 |
 Sheep anti-Human Tissue Factor polyclonal antibody |
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| CABT-L422 | Creative Diagnostics | 10 mg | EUR 663 |
Sheep anti-Human Tissue Factor polyclonal antibody |
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| CABT-L423 | Creative Diagnostics | 200ug | EUR 663 |
 Mouse anti-Human Tissue Factor monoclonal antibody |
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| CABT-L424 | Creative Diagnostics | 100ug | EUR 663 |
Mouse anti-Human Tissue Factor monoclonal antibody |
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| CABT-L425 | Creative Diagnostics | 100ug | EUR 663 |
) Tissue Factor antibody (HRP) |
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| 60R-1016 | Fitzgerald | 200 ug | EUR 358 |
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Description: Sheep polyclonal Tissue Factor antibody (HRP) conjugated |
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) Tissue Factor antibody (FITC) |
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| 60R-1056 | Fitzgerald | 100 ug | EUR 418 |
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Description: Sheep polyclonal Tissue Factor antibody (FITC) conjugated |
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) Tissue Factor antibody (biotin) |
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| 60R-1057 | Fitzgerald | 100 ug | EUR 418 |
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Description: Sheep polyclonal Tissue Factor antibody (biotin) conjugated |
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 Antibody) Tissue Factor (F3) Antibody |
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| abx117088-100ug | Abbexa | 100 ug | EUR 467 |
 Antibody) Tissue factor (F3) Antibody |
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| 20-abx323788 | Abbexa |
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Tissue factor (F3) Antibody |
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| 20-abx339467 | Abbexa |
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 Antibody) Tissue Factor (CD142) Antibody |
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| abx411647-01mg | Abbexa | 0.1 mg | EUR 439 |
Tissue Factor (CD142) Antibody |
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| abx414339-02mg | Abbexa | 0.2 mg | EUR 565 |
 Antibody) Tissue Factor (TF) Antibody |
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| 20-abx270078 | Abbexa |
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Tissue Factor (TF) Antibody |
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| 20-abx270129 | Abbexa |
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Tissue Factor (TF) Antibody |
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| 20-abx270230 | Abbexa |
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Tissue factor (F3) Antibody |
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| 20-abx210516 | Abbexa |
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Tissue Factor (TF) Antibody |
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| 20-abx178602 | Abbexa |
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Tissue Factor (TF) Antibody |
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| 20-abx178604 | Abbexa |
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Tissue Factor (TF) Antibody |
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| 20-abx174801 | Abbexa |
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Tissue Factor (TF) Antibody |
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| 20-abx174802 | Abbexa |
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Tissue Factor (TF) Antibody |
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| 20-abx174803 | Abbexa |
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Tissue Factor (F3) Antibody |
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| 20-abx303791 | Abbexa |
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Tissue factor (F3) Antibody |
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| 20-abx325045 | Abbexa |
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Tissue Factor (F3) Antibody |
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| 20-abx001211 | Abbexa |
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Tissue Factor (TF) Antibody |
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| 20-abx104870 | Abbexa |
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Tissue Factor (F3) Antibody |
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| 20-abx100873 | Abbexa |
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Tissue Factor (TF) Antibody |
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| 20-abx100874 | Abbexa |
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Tissue Factor (TF) Antibody |
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| 20-abx100875 | Abbexa |
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Tissue Factor (F3) Antibody |
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| 20-abx100876 | Abbexa |
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Tissue Factor (TF) Antibody |
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| 20-abx100877 | Abbexa |
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Tissue Factor (TF) Antibody |
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| 20-abx100878 | Abbexa |
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Tissue Factor (F3) Antibody |
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| abx037991-100ug | Abbexa | 100 ug | EUR 391 |
Tissue Factor (TF) Antibody |
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| 20-abx132340 | Abbexa |
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Tissue Factor (CD142) Antibody |
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| abx140398-01mg | Abbexa | 0.1 mg | EUR 356 |
Tissue Factor (CD142) Antibody |
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| 20-abx141195 | Abbexa |
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 Tissue Factor Conjugated Antibody |
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| C49112 | SAB | 100ul | EUR 397 |
 Polyclonal Goat anti-GST α-form |
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| GST-ANTI-1 | Detroit R&D | 50 uL | EUR 280 |
 Polyclonal Goat anti-GST μ-form |
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| GST-ANTI-2 | Detroit R&D | 50 uL | EUR 280 |
 Polyclonal Goat anti-GST p-form |
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| GST-ANTI-3 | Detroit R&D | 50 uL | EUR 280 |
 Polyclonal Goat Anti-Tissue Factor Pathway Inhibitor Antibody |
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| APG00335G | Leading Biology | 0.1mg | EUR 484 |
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Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-Tissue Factor Pathway Inhibitor . This antibody is tested and proven to work in the following applications: |
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 Mouse Monoclonal Anti-Human Tissue Factor IgG |
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| TIF11-M | Alpha Diagnostics | 100 ug | EUR 482 |
 Tissue Factor recombinant monoclonal antibody |
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| A5658 | Bimake | 100ul X 3 | EUR 595 |
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Description: A recombinant monoclonal antibody from rabbit against human Tissue Factor for WB, IHC, IF,ELISA |
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 Antibody Pair) Tissue Factor (TF) Antibody Pair |
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| 20-abx370347 | Abbexa |
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 Antibody (FITC)) Tissue Factor (CD142) Antibody (FITC) |
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| abx414337-01mg | Abbexa | 0.1 mg | EUR 509 |
Tissue Factor (CD142) Antibody (FITC) |
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| abx414338-25ug | Abbexa | 25 ug | EUR 272 |
 Antibody (FITC)) Tissue Factor (TF) Antibody (FITC) |
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| 20-abx270376 | Abbexa |
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Tissue Factor (TF) Antibody (FITC) |
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| 20-abx270427 | Abbexa |
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Tissue Factor (TF) Antibody (FITC) |
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| 20-abx270528 | Abbexa |
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 Antibody (APC)) Tissue Factor (TF) Antibody (APC) |
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| 20-abx270608 | Abbexa |
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Tissue Factor (TF) Antibody (APC) |
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| 20-abx270659 | Abbexa |
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Tissue Factor (TF) Antibody (APC) |
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| 20-abx270760 | Abbexa |
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 Antibody (PE)) Tissue Factor (TF) Antibody (PE) |
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| 20-abx270840 | Abbexa |
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Tissue Factor (TF) Antibody (PE) |
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| 20-abx270891 | Abbexa |
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Tissue Factor (TF) Antibody (PE) |
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| 20-abx270992 | Abbexa |
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Tissue Factor (TF) Antibody (APC) |
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| 20-abx178603 | Abbexa |
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Tissue Factor (TF) Antibody (FITC) |
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| 20-abx274001 | Abbexa |
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Tissue Factor (TF) Antibody (FITC) |
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| 20-abx274137 | Abbexa |
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 Antibody (Biotin)) Tissue Factor (TF) Antibody (Biotin) |
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| 20-abx271994 | Abbexa |
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Tissue Factor (TF) Antibody (Biotin) |
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| 20-abx272699 | Abbexa |
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Tissue Factor (TF) Antibody (Biotin) |
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| 20-abx273044 | Abbexa |
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Tissue Factor (TF) Antibody (Biotin) |
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| 20-abx273176 | Abbexa |
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Tissue Factor (TF) Antibody (FITC) |
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| 20-abx273794 | Abbexa |
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 Antibody (HRP)) Tissue Factor (F3) Antibody (HRP) |
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| 20-abx303792 | Abbexa |
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 Antibody (FITC)) Tissue Factor (F3) Antibody (FITC) |
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| 20-abx303793 | Abbexa |
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 Antibody (Biotin)) Tissue Factor (F3) Antibody (Biotin) |
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| 20-abx303794 | Abbexa |
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 Antibody) Human Tissue Factor (TF) Antibody |
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| 11611-05011 | AssayPro | 150 ug | EUR 217 |
 Antibody) Mouse Tissue Factor (TF) Antibody |
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| 32800-05111 | AssayPro | 150 ug | EUR 261 |
 Sheep anti-Human Tissue Factor Pathway Inhibitor polyclonal antibody |
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| CABT-L505 | Creative Diagnostics | 10 mg | EUR 663 |
) Recombinant Tissue Factor (TF) |
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| 4-RPA524Bo01 | Cloud-Clone |
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Description: Recombinant Bovine Tissue Factor expressed in: E.coli |
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Recombinant Tissue Factor (TF) |
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| 4-RPA524Hu01 | Cloud-Clone |
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Description: Recombinant Human Tissue Factor expressed in: E.coli |
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Recombinant Tissue Factor (TF) |
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| 4-RPA524Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Tissue Factor expressed in: E.coli |
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Recombinant Tissue Factor (TF) |
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| 4-RPA524Ra01 | Cloud-Clone |
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Description: Recombinant Rat Tissue Factor expressed in: E.coli |
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Recombinant Tissue Factor (TF) |
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| 4-RPA524Rb01 | Cloud-Clone |
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Description: Recombinant Rabbit Tissue Factor expressed in: E.coli |
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) Human Tissue factor (F3) |
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| 1-CSB-EP007928HU1 | Cusabio |
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Description: Recombinant Human Tissue factor(F3),partial expressed in E.coli |
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) Active Tissue Factor (TF) |
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| 4-APA524Ra01 | Cloud-Clone |
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Description: Recombinant Rat Tissue Factor expressed in: E.coli |
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 Protein) Tissue Factor (F3) Protein |
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| 20-abx262300 | Abbexa |
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 Tissue Factor Rabbit mAb |
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| A4395-100ul | Abclonal | 100 ul | EUR 410 |
Tissue Factor Rabbit mAb |
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| A4395-200ul | Abclonal | 200 ul | EUR 571 |
Tissue Factor Rabbit mAb |
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| A4395-20ul | Abclonal | 20 ul | EUR 221 |
Tissue Factor Rabbit mAb |
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| A4395-50ul | Abclonal | 50 ul | EUR 287 |
 CD142/Tissue factor/F3 |
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| E21-479 | EnoGene | 10ug | EUR 343 |
 Monoclonal Antibody (Human)) Tissue Factor (TF) Monoclonal Antibody (Human) |
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| 4-MAA524Hu21 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Tissue Factor (TF) |
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Tissue Factor (TF) Monoclonal Antibody (Human) |
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| 4-MAA524Hu22 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Tissue Factor (TF) |
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 Monoclonal Antibody (Mouse)) Tissue Factor (TF) Monoclonal Antibody (Mouse) |
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| 4-MAA524Mu21 | Cloud-Clone |
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Description: A Rat monoclonal antibody against Mouse Tissue Factor (TF) |
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 Monoclonal Antibody (Rat)) Tissue Factor (TF) Monoclonal Antibody (Rat) |
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| 4-MAA524Ra21 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rat Tissue Factor (TF) |
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 Monoclonal Antibody (Rabbit)) Tissue Factor (TF) Monoclonal Antibody (Rabbit) |
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| 4-MAA524Rb21 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rabbit Tissue Factor (TF) |
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 Polyclonal Antibody (Bovine)) Tissue Factor (TF) Polyclonal Antibody (Bovine) |
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| 4-PAA524Bo01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Bovine Tissue Factor (TF) |
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 Polyclonal Antibody (Human)) Tissue Factor (TF) Polyclonal Antibody (Human) |
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| 4-PAA524Hu01 | Cloud-Clone |
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|
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Description: A Rabbit polyclonal antibody against Human Tissue Factor (TF) |
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 Polyclonal Antibody (Mouse)) Tissue Factor (TF) Polyclonal Antibody (Mouse) |
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| 4-PAA524Mu01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Tissue Factor (TF) |
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 Polyclonal Antibody (Rat)) Tissue Factor (TF) Polyclonal Antibody (Rat) |
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| 4-PAA524Ra01 | Cloud-Clone |
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|
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Description: A Rabbit polyclonal antibody against Rat Tissue Factor (TF) |
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 Polyclonal Antibody (Rabbit)) Tissue Factor (TF) Polyclonal Antibody (Rabbit) |
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| 4-PAA524Rb51 | Cloud-Clone |
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Description: A Guinea polyclonal antibody against Rabbit Tissue Factor (TF) |
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 Antibody) Connective Tissue Growth Factor (CTGF) Antibody |
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| 20-abx125726 | Abbexa |
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Connective Tissue Growth Factor (CTGF) Antibody |
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| 20-abx125727 | Abbexa |
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Connective Tissue Growth Factor (CTGF) Antibody |
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| 20-abx125728 | Abbexa |
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 Antibody) Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| 20-abx129517 | Abbexa |
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Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| 20-abx116110 | Abbexa |
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Connective Tissue Growth Factor (CTGF) Antibody |
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| 20-abx109670 | Abbexa |
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Connective Tissue Growth Factor (CTGF) Antibody |
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| 20-abx123700 | Abbexa |
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 Antibody) Connective Tissue Growth Factor (Ctgf) Antibody |
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| 20-abx319302 | Abbexa |
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Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| 20-abx323593 | Abbexa |
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Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| abx433382-200ul | Abbexa | 200 ul | EUR 384 |
Connective Tissue Growth Factor (CTGF) Antibody |
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| abx433502-200ul | Abbexa | 200 ul | EUR 453 |
Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| abx238634-100ug | Abbexa | 100 ug | EUR 551 |
 Antibody) Connective tissue growth factor (CTGF) Antibody |
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| 20-abx214776 | Abbexa |
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Connective Tissue Growth Factor (CTGF) Antibody |
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| abx232053-100ug | Abbexa | 100 ug | EUR 509 |
Connective Tissue Growth Factor (CTGF) Antibody |
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| abx232054-100ug | Abbexa | 100 ug | EUR 509 |
Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| 20-abx174804 | Abbexa |
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Connective Tissue Growth Factor (CTGF) Antibody |
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| 20-abx175995 | Abbexa |
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Connective Tissue Growth Factor (CTGF) Antibody |
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| 20-abx175996 | Abbexa |
|
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Connective Tissue Growth Factor (CTGF) Antibody |
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| 20-abx175997 | Abbexa |
|
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Connective Tissue Growth Factor (CTGF) Antibody |
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| 20-abx171909 | Abbexa |
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Connective Tissue Growth Factor (CTGF) Antibody |
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| 20-abx171910 | Abbexa |
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Connective Tissue Growth Factor (CTGF) Antibody |
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| 20-abx225128 | Abbexa |
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Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| 20-abx001368 | Abbexa |
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Connective Tissue Growth Factor (CTGF) Antibody |
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| 20-abx103225 | Abbexa |
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Connective Tissue Growth Factor (CTGF) Antibody |
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| 20-abx103226 | Abbexa |
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Connective Tissue Growth Factor (CTGF) Antibody |
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| 20-abx103227 | Abbexa |
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Connective Tissue Growth Factor (CTGF) Antibody |
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| 20-abx103228 | Abbexa |
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Connective Tissue Growth Factor (CTGF) Antibody |
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| 20-abx103229 | Abbexa |
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Connective Tissue Growth Factor (CTGF) Antibody |
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| 20-abx103230 | Abbexa |
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Connective Tissue Growth Factor (CTGF) Antibody |
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| 20-abx103231 | Abbexa |
|
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Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| 20-abx103325 | Abbexa |
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Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| 20-abx103326 | Abbexa |
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Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| 20-abx103328 | Abbexa |
|
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Exosomes are rising as a promising strategy for treating peripheral nerve harm. Moreover, additionally they present potentialities for enhancing the restore capability of varied nerve grafts. This evaluation primarily highlights the regenerative results of exosomes on peripheral nerve harm. The exosomes from distinct sources reported up to now in literature are summarized to know their roles within the technique of nerve restore.
Furthermore, the challenges that should be addressed of their scientific transformation are outlined as nicely. This evaluation additionally offers additional perception into the potential utility of exosomes for peripheral nerve restore. Key phrases: Exosome, nerve regeneration, peripheral nerve harm, Schwann cell, axonal regrowth, irritation, vascular regeneration.