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Cell sheet technology: Influence of culture conditions on in vitro-cultivated corneal stromal tissue for regenerative therapies of the ocular surface

Cell sheet technology: Influence of culture conditions on in vitro-cultivated corneal stromal tissue for regenerative therapies of the ocular surface

The in vitro reconstruction of stromal tissue by long-term cultivation of corneal fibroblasts is a brilliant method for regenerative therapies of ocular floor ailments. Nevertheless, systematic investigations evaluating optimized cultivation protocols for the conclusion of a biomaterial are missing. This examine investigated the affect of dietary supplements to the tradition media of human corneal fibroblasts on the formation of a cell sheet consisting of cells and extracellular matrix. Among the many dietary supplements studied are vitamin C, fetal bovine serum, L-glutamine, elements of collagen reminiscent of L-proline, L-4-hydroxyproline and glycine, and TGF-β1, bFGF, IGF-2, PDGF-BB and insulin. After long-term cultivation, the proliferation, collagen and glycosaminoglycan content material and lightweight transmission of the cell sheets have been examined.

Biomechanical properties have been investigated by tensile exams and the ultrastructure was characterised by electron microscopy, small-angle X-ray scattering, antibody staining and ELISA. The synthesis of extracellular matrix was considerably elevated by cultivation with insulin or TGF-β1, every with vitamin C. The sheets exhibited a excessive transparency and appropriate materials properties. The manufacturing of a clear, scaffold-free, probably autologous, in vitro-generated assemble by culturing fibroblasts with extracellular matrix synthesis-stimulating dietary supplements represents a promising method for a biomaterial that can be utilized for ocular floor reconstruction in slowly progressing ailments.

Bioengineered 3D tunable neuronal constructs are a flexible platform for learning neuronal community features, providing quite a few benefits over current applied sciences and offering for the invention of latest organic insights. Practical neural networks could be evaluated utilizing calcium imaging and quantitatively described utilizing community science. This protocol contains directions for fabricating protein-based composite scaffolds, 3D in vitro tradition of embryonic mouse cortical neurons, virally induced expression of GCaMP6f, wide-field calcium imaging, and computational evaluation with open-source software program and customized MATLAB code. For full particulars on the use and execution of this protocol, please discuss with Dingle et al. (2020).

Mechanobiology in Tendon, Ligament, and Skeletal Muscle Tissue Engineering

Tendon, ligament, and skeletal muscle are highly-organized tissues that largely depend on a hierarchical collagenous matrix to face up to excessive tensile hundreds skilled in actions of every day life. This essential biomechanical position predisposes these tissues to damage, and present therapies fail to recapitulate the biomechanical perform of native tissue. This has prompted researchers to pursue engineering purposeful tissue replacements, or dysfunction/illness/improvement fashions, by emulating in vivo stimuli inside in vitro tissue engineering platforms; particularly mechanical stimulation, in addition to energetic contraction in skeletal muscle. Mechanical loading is essential for matrix manufacturing and group within the improvement, maturation, and upkeep of native tendon, ligament, and skeletal muscle, in addition to their interfaces.
Tissue engineers search to harness these mechanobiological advantages utilizing bioreactors to use each static and dynamic mechanical stimulation to tissue constructs, and induce energetic contraction in engineered skeletal muscle. The overwhelming majority of engineering approaches in these tissues are scaffold-based, offering interim construction and help to engineered constructs, and ample integrity to face up to mechanical loading. Alternatively, some latest research have employed developmentally-inspired scaffold-free strategies, counting on mobile self-assembly and matrix manufacturing to kind tissue constructs. Whether or not using a scaffold or not, incorporation of mechanobiological stimuli has been proven to enhance the composition, construction, and biomechanical perform of engineered tendon, ligament, and skeletal muscle. Collectively, these findings spotlight the significance of mechanobiology and recommend how it may be leveraged to engineer these tissues and their interfaces, and to create purposeful multi-tissue constructs.
Cell sheet technology: Influence of culture conditions on in vitro-cultivated corneal stromal tissue for regenerative therapies of the ocular surface

Biomimetic Mineralization Promotes Viability and Differentiation of Human Mesenchymal Stem Cells in a Perfusion Bioreactor

In bone tissue engineering, the design of 3D programs able to recreating composition, structure and micromechanical atmosphere of the native extracellular matrix (ECM) continues to be a problem. Whereas perfusion bioreactors have been proposed as potential device to use biomechanical stimuli, its use has been restricted to a low variety of biomaterials. On this work, we suggest the tradition of human mesenchymal stem cells (hMSC) in biomimetic mineralized recombinant collagen scaffolds with a perfusion bioreactor to concurrently present biochemical and biophysical cues guiding stem cell destiny.
The scaffolds have been fabricated by mineralization of recombinant collagen within the presence of magnesium (RCP.MgAp). The natural matrix was homogeneously mineralized with apatite nanocrystals, comparable in composition to these present in bone. X-Ray microtomography photographs revealed isotropic porous construction with optimum porosity for cell ingrowth. In actual fact, an optimum cell repopulation via the whole scaffolds was obtained after 1 day of dynamic seeding within the bioreactor. Remarkably, RCP.MgAp scaffolds exhibited larger cell viability and a transparent development of up-regulation of osteogenic genes than management (non-mineralized) scaffolds. Outcomes reveal the potential of the mix of biomimetic mineralization of recombinant collagen in presence of magnesium and dynamic tradition of hMSC as a promising technique to carefully mimic bone ECM.

cDNA - Mouse Normal Tissue: Kidney

C1334142 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Kidney

C1234142 40 reactions
EUR 424

cDNA - Monkey (Rhesus) Normal Tissue: Kidney

C1534142 40 reactions
EUR 481

cDNA - Monkey (Cynomolgus) Normal Tissue: Kidney

C1534142-Cy 40 reactions
EUR 481

Total RNA - Rat Normal Tissue: Kidney

R1434142-50 50 ug
EUR 156

Genomic DNA - Rat Normal Tissue: Kidney

D1434142 100 ug
EUR 329

Rat normal kidney tissue array

TR071a each
EUR 48
Description: Rat normal kidney tissue array, 6 cases/24 cores, replacing TR071

Normal kidney and normal adjacent kidney tissue array

KDN241a each
EUR 138
Description: Normal kidney and normal adjacent kidney tissue array, 24 cases/ 24 cores

Matched Pair - cDNA - Human Primary Tumor and Normal Tissue: Kidney

C8235142-PP 10 reactions x2
EUR 583

Kidney cancer tissue array with normal Kidney tissue

KD2082a each
EUR 474
Description: Kidney cancer tissue array with normal Kidney tissue, including TNM and pathology grade, 96 cases/192 cores, replacing KD2082

Kidney Tissue Slide (Normal)

10-401-10um 10 um
EUR 241.8

Kidney Tissue Slide (Normal)

10-401-4um 4 um
EUR 216.6

Normal kidney tissue array

KD803 each
EUR 354
Description: Normal kidney tissue array, replacing BN07012, 80 cases/80 cores

Normal kidney tissue array

KDN242 each
EUR 72
Description: Normal kidney tissue array, 24 cases/24 cores

Kidney Tissue Lysate (Normal)

1706-02 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-03 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-04 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-05 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Rat Tissue Lysate Kidney Medulla Normal

GWB-F5DEBF 0.15 mg Ask for price

Kidney cancer tissue array with adjacent normal kidney tissue

BC07015b each
EUR 168
Description: Kidney cancer tissue array with adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 72 cases/72 cores, replacing BC07015a

Total RNA - Mouse Normal Tissue: Kidney

R1334142-50 50 ug
EUR 156

Genomic DNA - Mouse Normal Tissue: Kidney

D1334142 100 ug
EUR 329

Total Protein - Mouse Normal Tissue: Kidney

P1334142 1 mg
EUR 216

Tissue Lysate (Normal) Human Kidney

GWB-FEAC28 0.15 mg Ask for price

Kidney cancer tissue array with matched adjacent normal kidney tissue

KD901a each
EUR 258
Description: Kidney cancer tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 40 cases/90 cores, replacing KD901

Kidney tumour with normal tissue array

KD2001 each
EUR 474
Description: Kidney tumour with normal tissue array, including Clear cell carcinoma (CCRCC), Sarcomatoid carcinoma, Papillary renal cell carcinoma (PRCC), Chromopobe carcinoma(crcc), Invasive low grade urothelial carcinoma, with Isup Grade, TNM/Stage (AJCC 8th edition), 100 cases/200 cores (core size 1.0mm),replacing BC07114

Total RNA - Human Adult Normal Tissue: Kidney

R1234142-50 50 ug
EUR 221

Kidney cancer tissue array with normal tissues

KD2083 each
EUR 474
Description: Kidney cancer tissue array with normal tissues, with stage and grade info, 69 cases/208 cores

Kidney cancer tissue array with normal tissues

KD721 each
EUR 168
Description: Kidney cancer tissue array with normal tissues, 24 cases/72 cores

Mouse Normal Tissue: Kidney Whole Cell Lysate

MNK-100 1 mg
EUR 628.8

Mouse Normal Tissue: Kidney Whole Cell Lysate

MNK-50 50 ug
EUR 196.8

Total RNA - Monkey (Rhesus) Normal Tissue: Kidney

R1534142-50 50 ug
EUR 229

Kidney tumor tissue array with normal tissue as control

KD961 each
EUR 258
Description: Kidney tumor tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores

Total Protein - Human Adult Normal Tissue: Kidney

P1234142 1 mg
EUR 216

Kidney cancer tissue array with normal tissue as control

KD962 each
EUR 258
Description: Kidney cancer tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores

Rat Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80263 1 x 100 ml Ask for price

Total RNA - Monkey (Cynomolgus) Normal Tissue: Kidney

R1534142-Cy 50 ug
EUR 229

Adjacent normal kidney tissue and cancer tissue array

BN07011b each
EUR 203
Description: Adjacent normal kidney tissue and cancer tissue array, including pathology grade, TNM and clinical stage, 20 cases/54 cores, replacing BN07011

Membrane Protein - Human Adult Normal Tissue: Kidney

P3234142 0.1 mg
EUR 311

Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue

KD701a each
EUR 234
Description: Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 35 cases/70 cores, replacing KD701

FFPE Total RNA - Human Adult Normal Tissue: Kidney

R2234142 1 ug
EUR 744

Kidney cancer tissue array with matched adjacent normal tissue

KD321a each
EUR 198
Description: Kidney cancer tissue array with matched adjacent normal tissue, including TNM, clinical stage and pathology ISUP grade, 16 cases/32 cores, replacing KD321

Kidney cancer with matched adjacent normal kidney tissue array

KD603 each
EUR 204
Description: Kidney cancer with matched adjacent normal kidney tissue array, containing metastatic clear cell carcinoma, including pathology grade, TNM and clinical stage (reference AJCC 8th version), 33 cases/61 cores

Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue

Kid-060Sur01 each
EUR 954
Description: Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue, including TNM, ISUP grade and survival data, 30 cases/60 cores

Human Adult Kidney (Normal) Whole tissue lysate

HAL-1305 1 mg
EUR 628.8

Monkey (Cyno.) Normal Kidney Whole tissue lysate

MCL-1190 1 mg
EUR 628.8

Monkey (Rhesus) Normal Kidney Whole tissue lysate

MRL-1301 1 mg
EUR 628.8

Frozen Tissue Section - Human Adult Normal: Kidney

T1234142 5 slides
EUR 377

Kidney Tumor Tissue Array - 12 cases of kidney cancer paired with adjacent normal tissues

Z7020053 5 slides
EUR 1125.6
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Adult Normal: Kidney

T2234142 5 slides
EUR 204

Human Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80075 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80171 1 x 100 ml Ask for price

Multiple Kidney cancer tissue array with normal tissue control from autopsy

KD483 each
EUR 168
Description: Multiple Kidney cancer tissue array with normal tissue control from autopsy, 48 cases/48 cores, with stage and grade data

Matched Pair - DNA - Human Primary Tumor and Normal Tissue: Kidney

D8235142-PP-10 2x10 ug
EUR 478

Rat Kidney Tissue Preparation Buffer 6: Normal Renal Firbroblasts

9-80267 1 x 100 ml Ask for price

Total RNA - Human Adult Normal Tissue 5 Donor Pool: Kidney

R1234142-P 50 ug
EUR 443

Rat Normal Kidney tissue (single section per slide) (5 slides/pack)

RaFPT007 5 slides
EUR 150

Kidney Tissue Slides, Normal Rat Paraffin Sections, 5 slides/pack

TS-R5405 -
EUR 145

High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue

KD208 each
EUR 546
Description: High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue

Genomic DNA - Human Adult Normal Tissue: Kidney, from a single donor

D1234142 100 ug
EUR 329

Matched Pair - Total RNA - Human Primary Tumor and Normal Tissue: Kidney

R8235142-PP-10 2x10 ug
EUR 500

Mouse Kidney Tissue Preparation Buffer 3: Normal Podocytes

9-80172 1 x 100 ml Ask for price

cDNA - Rat Normal Tissue: Lung

C1434152 40 reactions
EUR 424

Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue

KD242 each
EUR 48
Description: Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue, 10 cases/24 cores

cDNA - Rat Normal Tissue: Brain

C1434035 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Colon

C1434090 40 reactions
EUR 636

cDNA - Rat Normal Tissue: Heart

C1434122 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Liver

C1434149 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Rectum

C1434206 40 reactions
EUR 636

cDNA - Rat Normal Tissue: Spleen

C1434246 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Testis

C1434260 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Adipose

C1434003 40 reactions
EUR 636

cDNA - Rat Normal Tissue: Bladder

C1434010 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Stomach

C1434248 40 reactions
EUR 424

Kidney cancer with matched cancer adjacent normal tissue test array

KD241 each
EUR 48
Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores

Kidney cancer with matched cancer adjacent normal tissue test array

KD241a each
EUR 66
Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores, replacing KD241

cDNA - Rat Normal Tissue: Placenta

C1434200 40 reactions
EUR 636

Rat Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells

9-80262 1 x 100 ml Ask for price

Matched Pair - Total Protein - Human Primary Tumor and Normal Tissue (PP): Kidney

P8235142-PP 0.2 mg x2
EUR 716

Human Kidney Tissue Preparation Buffer 6: Normal Renal Podocytes

9-80079 1 x 100 ml Ask for price

Rat Kidney Tissue Preparation Buffer 5: Normal Renal Artery Endothelial Cells

9-80266 1 x 100 ml Ask for price

Rat Kidney Tissue Preparation Buffer 8: Normal Renal Tubular Epithelial Cells

9-80269 1 x 100 ml Ask for price

Human Kidney Tissue Preparation Buffer 4: Normal Renal Firbroblasts

9-80077 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 5: Normal Renal Firbroblasts

9-80174 1 x 100 ml Ask for price

Kidney cancer tissue array with matched normal adjacent tissue and metastatic carcinoma

KD951a each
EUR 1434
Description: Kidney cancer tissue array with matched normal adjacent tissue and metastatic carcinoma, with TNM, clinical stage, pathology grade and survival data, 32 cases/95 cores,replacing KD951

Kidney Tissue Slides, Normal Human Paraffin Sections, 5 slides/pack

TS-H5013 -
EUR 145

Kidney Tissue Slides, Normal Mouse Paraffin Sections, 5 slides/pack

TS-M5203 -
EUR 145

Human Kidney Tissue Preparation Buffer 5: Normal Renal Mesangial Cells

9-80078 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 6: Normal Renal Mesangial Cells

9-80175 1 x 100 ml Ask for price

cDNA - Rat Normal Tissue: Skeletal Muscle

C1434171 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Small Intestine

C1434226 40 reactions
EUR 636

cDNA - Human Tumor Tissue: Kidney

C1235142 40 reactions
EUR 636

Matched Pair (Normal and Carcinoma) Human Kidney Tissue Array (5 slides/pk)

TAS-1007 1 pk
EUR 343.2

30 case of Kidney clear cell carcinoma with 5 of NAT and 5 normal tissue array

KD805 each
EUR 258
Description: 30 case of Kidney clear cell carcinoma with 5 of NAT and 5 normal tissue array, 40 cases/80 cores

Human Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells

9-80074 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells

9-80170 1 x 100 ml Ask for price

Matched Pair - Frozen Tissue Section - Human Primary Tumor and Normal (PP): Kidney

T6235142-PP 5 slides x2
EUR 595

Paired Cancer and Normal Kidney Paraffin Tissue (FFPE) Section (5 slides/pack)

NCT176 5 slides
EUR 300

Human Kidney Tissue Preparation Buffer 3: Normal Renal Artery Endothelial Cells

9-80076 1 x 100 ml Ask for price

Human Kidney Tissue Preparation Buffer 7: Normal Renal Tubular Epithelial Cells

9-80080 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 4: Normal Renal Artery Endothelial Cells

9-80173 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 8: Normal Renal Tubular Epithelial Cells

9-80177 1 x 100 ml Ask for price

Matched Pair - Paraffin Tissue Section - Human Primary Tumor and Normal (PP): Kidney

T8235142-PP 5 slides x2
EUR 383

cDNA - Dog Normal Tissue: Lung

C1734152 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Brain

C1734035 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Cecum

C1734089 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Heart

C1734122 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Liver

C1734149 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Lung

C1334152 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Rectum

C1734206 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Testis

C1734260 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Brain

C1334035 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Colon

C1334090 40 reactions
EUR 636

cDNA - Mouse Normal Tissue: Heart

C1334122 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Liver

C1334149 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Bladder

C1734010 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Trachea

C1734160 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Stomach

C1734248 40 reactions
EUR 424

Frozen Tissue Array - 37 Different Kidney Tumors and 3 Corresponding Normal Controls

T6235142-1 1 slide
EUR 761

Frozen Tissue Array - 37 Different Kidney Tumors and 3 Corresponding Normal Controls

T6235142-5 5 slides
EUR 2500

cDNA - Mouse Normal Tissue: Rectum

C1334206 40 reactions
EUR 636

cDNA - Mouse Normal Tissue: Spleen

C1334246 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Testis

C1334260 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Pancreas

C1734188 40 reactions
EUR 424

cDNA - Plant Normal Tissue: cDNA - Plant: Corn

C1634330 40 reactions
EUR 741

cDNA - Plant Normal Tissue: cDNA - Plant: Rice

C1634360 40 reactions
EUR 741

cDNA - Plant Normal Tissue: cDNA - Plant: Wheat

C1634390 40 reactions
EUR 741

cDNA - Mouse Normal Tissue: Adipose

C1334003 40 reactions
EUR 636

cDNA - Mouse Normal Tissue: Bladder

C1334010 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Stomach

C1334248 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Esophagus

C1734106 40 reactions
EUR 424

cDNA - Human Diabetic Diseased Tissue: Kidney

C1236142Dia 40 reactions
EUR 802

cDNA - Plant Normal Tissue: cDNA - Plant: Orange

C1634340 40 reactions
EUR 741

cDNA - Plant Normal Tissue: cDNA - Plant: Potato

C1634350 40 reactions
EUR 741

cDNA - Mouse Normal Tissue: Placenta

C1334200 40 reactions
EUR 636

Kidney Tumor Tissue Array - Duplicated 70 cases covering all the common types of kidney cancer and 5 cases of normal and

Z7020052 5 slides
EUR 1657.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Kidney Tumor Tissue Array - Duplicated 36 cases covering all the common types of kidney cancer and 12 cases of normal an

Z7020054 5 slides
EUR 1657.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

cDNA - Plant Normal Tissue: cDNA - Plant: Arabidopsis

C1634310 40 reactions
EUR 741

cDNA - Plant Normal Tissue: cDNA - Plant: Soy bean

C1634370 40 reactions
EUR 741

cDNA - Human Adult Normal Tissue: Lung

C1234152 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Skin

C1234218 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Brain

C1234035 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Colon

C1234090 40 reactions
EUR 424
Tandem Mass Tag quantitative proteomics was utilized to disclose the temporal protein expression sample throughout osteogenic differentiation of hPDLSCs on days 0, 3, 7 and 14. Differentially expressed proteins (DEPs) have been clustered and purposeful annotated by Gene Ontology (GO) phrases. Pathway enrichment evaluation was carried out based mostly on the Kyoto Encyclopedia of Genes and Genomes database, adopted by the anticipated activation utilizing Ingenuity Pathway Evaluation software program. Interplay networks of redox-sensitive signalling pathways and oxidative phosphorylation (OXPHOS) have been performed and the hub protein SOD2 was validated with western blotting.

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