The in vitro reconstruction of stromal tissue by long-term cultivation of corneal fibroblasts is a brilliant method for regenerative therapies of ocular floor ailments. Nevertheless, systematic investigations evaluating optimized cultivation protocols for the conclusion of a biomaterial are missing. This examine investigated the affect of dietary supplements to the tradition media of human corneal fibroblasts on the formation of a cell sheet consisting of cells and extracellular matrix. Among the many dietary supplements studied are vitamin C, fetal bovine serum, L-glutamine, elements of collagen reminiscent of L-proline, L-4-hydroxyproline and glycine, and TGF-β1, bFGF, IGF-2, PDGF-BB and insulin. After long-term cultivation, the proliferation, collagen and glycosaminoglycan content material and lightweight transmission of the cell sheets have been examined.
Biomechanical properties have been investigated by tensile exams and the ultrastructure was characterised by electron microscopy, small-angle X-ray scattering, antibody staining and ELISA. The synthesis of extracellular matrix was considerably elevated by cultivation with insulin or TGF-β1, every with vitamin C. The sheets exhibited a excessive transparency and appropriate materials properties. The manufacturing of a clear, scaffold-free, probably autologous, in vitro-generated assemble by culturing fibroblasts with extracellular matrix synthesis-stimulating dietary supplements represents a promising method for a biomaterial that can be utilized for ocular floor reconstruction in slowly progressing ailments.
Bioengineered 3D tunable neuronal constructs are a flexible platform for learning neuronal community features, providing quite a few benefits over current applied sciences and offering for the invention of latest organic insights. Practical neural networks could be evaluated utilizing calcium imaging and quantitatively described utilizing community science. This protocol contains directions for fabricating protein-based composite scaffolds, 3D in vitro tradition of embryonic mouse cortical neurons, virally induced expression of GCaMP6f, wide-field calcium imaging, and computational evaluation with open-source software program and customized MATLAB code. For full particulars on the use and execution of this protocol, please discuss with Dingle et al. (2020).
Mechanobiology in Tendon, Ligament, and Skeletal Muscle Tissue Engineering
Tendon, ligament, and skeletal muscle are highly-organized tissues that largely depend on a hierarchical collagenous matrix to face up to excessive tensile hundreds skilled in actions of every day life. This essential biomechanical position predisposes these tissues to damage, and present therapies fail to recapitulate the biomechanical perform of native tissue. This has prompted researchers to pursue engineering purposeful tissue replacements, or dysfunction/illness/improvement fashions, by emulating in vivo stimuli inside in vitro tissue engineering platforms; particularly mechanical stimulation, in addition to energetic contraction in skeletal muscle. Mechanical loading is essential for matrix manufacturing and group within the improvement, maturation, and upkeep of native tendon, ligament, and skeletal muscle, in addition to their interfaces.
Tissue engineers search to harness these mechanobiological advantages utilizing bioreactors to use each static and dynamic mechanical stimulation to tissue constructs, and induce energetic contraction in engineered skeletal muscle. The overwhelming majority of engineering approaches in these tissues are scaffold-based, offering interim construction and help to engineered constructs, and ample integrity to face up to mechanical loading. Alternatively, some latest research have employed developmentally-inspired scaffold-free strategies, counting on mobile self-assembly and matrix manufacturing to kind tissue constructs. Whether or not using a scaffold or not, incorporation of mechanobiological stimuli has been proven to enhance the composition, construction, and biomechanical perform of engineered tendon, ligament, and skeletal muscle. Collectively, these findings spotlight the significance of mechanobiology and recommend how it may be leveraged to engineer these tissues and their interfaces, and to create purposeful multi-tissue constructs.
Biomimetic Mineralization Promotes Viability and Differentiation of Human Mesenchymal Stem Cells in a Perfusion Bioreactor
In bone tissue engineering, the design of 3D programs able to recreating composition, structure and micromechanical atmosphere of the native extracellular matrix (ECM) continues to be a problem. Whereas perfusion bioreactors have been proposed as potential device to use biomechanical stimuli, its use has been restricted to a low variety of biomaterials. On this work, we suggest the tradition of human mesenchymal stem cells (hMSC) in biomimetic mineralized recombinant collagen scaffolds with a perfusion bioreactor to concurrently present biochemical and biophysical cues guiding stem cell destiny.
The scaffolds have been fabricated by mineralization of recombinant collagen within the presence of magnesium (RCP.MgAp). The natural matrix was homogeneously mineralized with apatite nanocrystals, comparable in composition to these present in bone. X-Ray microtomography photographs revealed isotropic porous construction with optimum porosity for cell ingrowth. In actual fact, an optimum cell repopulation via the whole scaffolds was obtained after 1 day of dynamic seeding within the bioreactor. Remarkably, RCP.MgAp scaffolds exhibited larger cell viability and a transparent development of up-regulation of osteogenic genes than management (non-mineralized) scaffolds. Outcomes reveal the potential of the mix of biomimetic mineralization of recombinant collagen in presence of magnesium and dynamic tradition of hMSC as a promising technique to carefully mimic bone ECM.
 cDNA - Mouse Normal Tissue: Kidney |
|
C1334142 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Human Adult Normal Tissue: Kidney |
|
C1234142 |
Biochain |
40 reactions |
EUR 385 |
 Normal Tissue: Kidney) cDNA - Monkey (Rhesus) Normal Tissue: Kidney |
|
C1534142 |
Biochain |
40 reactions |
EUR 385 |
 Normal Tissue: Kidney) cDNA - Monkey (Cynomolgus) Normal Tissue: Kidney |
|
C1534142-Cy |
Biochain |
40 reactions |
EUR 385 |
 Matched Pair - cDNA - Human Primary Tumor and Normal Tissue: Kidney |
|
C8235142-PP |
Biochain |
10 reactions x2 |
EUR 530 |
 Total RNA - Rat Normal Tissue: Kidney |
|
R1434142-50 |
Biochain |
50 ug |
EUR 130 |
 Genomic DNA - Rat Normal Tissue: Kidney |
|
D1434142 |
Biochain |
100 ug |
EUR 286 |
 Rat normal kidney tissue array |
|
TR071a |
TissueArray |
each |
EUR 48 |
|
Description: Rat normal kidney tissue array, 6 cases/24 cores, replacing TR071 |
 Normal kidney and normal adjacent kidney tissue array |
|
KDN241a |
TissueArray |
each |
EUR 138 |
|
Description: Normal kidney and normal adjacent kidney tissue array, 24 cases/ 24 cores |
 Kidney cancer tissue array with normal Kidney tissue |
|
KD2082a |
TissueArray |
each |
EUR 474 |
|
Description: Kidney cancer tissue array with normal Kidney tissue, including TNM and pathology grade, 96 cases/192 cores, replacing KD2082 |
) Kidney Tissue Slide (Normal) |
|
10-401-10um |
ProSci |
10 um |
EUR 241.8 |
Kidney Tissue Slide (Normal) |
|
10-401-4um |
ProSci |
4 um |
EUR 216.6 |
 Normal kidney tissue array |
|
KD803 |
TissueArray |
each |
EUR 354 |
|
Description: Normal kidney tissue array, replacing BN07012, 80 cases/80 cores |
Normal kidney tissue array |
|
KDN242 |
TissueArray |
each |
EUR 72 |
|
Description: Normal kidney tissue array, 24 cases/24 cores |
) Kidney Tissue Lysate (Normal) |
|
1706-02 |
ProSci |
0.1 mg |
EUR 260.7 |
|
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Kidney Tissue Lysate (Normal) |
|
1706-03 |
ProSci |
0.1 mg |
EUR 260.7 |
|
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Kidney Tissue Lysate (Normal) |
|
1706-04 |
ProSci |
0.1 mg |
EUR 260.7 |
|
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Kidney Tissue Lysate (Normal) |
|
1706-05 |
ProSci |
0.1 mg |
EUR 260.7 |
|
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
 Kidney cancer tissue array with adjacent normal kidney tissue |
|
BC07015b |
TissueArray |
each |
EUR 168 |
|
Description: Kidney cancer tissue array with adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 72 cases/72 cores, replacing BC07015a |
 Total RNA - Mouse Normal Tissue: Kidney |
|
R1334142-50 |
Biochain |
50 ug |
EUR 130 |
 Genomic DNA - Mouse Normal Tissue: Kidney |
|
D1334142 |
Biochain |
100 ug |
EUR 286 |
 Total Protein - Mouse Normal Tissue: Kidney |
|
P1334142 |
Biochain |
1 mg |
EUR 188 |
 cDNA - Rat Normal Tissue: Lung |
|
C1434152 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Rat Normal Tissue: Brain |
|
C1434035 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Rat Normal Tissue: Colon |
|
C1434090 |
Biochain |
40 reactions |
EUR 578 |
 cDNA - Rat Normal Tissue: Heart |
|
C1434122 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Rat Normal Tissue: Liver |
|
C1434149 |
Biochain |
40 reactions |
EUR 385 |
 Kidney cancer tissue array with matched adjacent normal kidney tissue |
|
KD901a |
TissueArray |
each |
EUR 258 |
|
Description: Kidney cancer tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 40 cases/90 cores, replacing KD901 |
 Kidney tumour with normal tissue array |
|
KD2001 |
TissueArray |
each |
EUR 474 |
|
Description: Kidney tumour with normal tissue array, including Clear cell carcinoma (CCRCC), Sarcomatoid carcinoma, Papillary renal cell carcinoma (PRCC), Chromopobe carcinoma(crcc), Invasive low grade urothelial carcinoma, with Isup Grade, TNM/Stage (AJCC 8th edition), 100 cases/200 cores (core size 1.0mm),replacing BC07114 |
 cDNA - Rat Normal Tissue: Rectum |
|
C1434206 |
Biochain |
40 reactions |
EUR 578 |
 cDNA - Rat Normal Tissue: Spleen |
|
C1434246 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Rat Normal Tissue: Testis |
|
C1434260 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Rat Normal Tissue: Adipose |
|
C1434003 |
Biochain |
40 reactions |
EUR 578 |
 cDNA - Rat Normal Tissue: Bladder |
|
C1434010 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Rat Normal Tissue: Stomach |
|
C1434248 |
Biochain |
40 reactions |
EUR 385 |
 Total RNA - Human Adult Normal Tissue: Kidney |
|
R1234142-50 |
Biochain |
50 ug |
EUR 170 |
 cDNA - Rat Normal Tissue: Placenta |
|
C1434200 |
Biochain |
40 reactions |
EUR 578 |
 Kidney cancer tissue array with normal tissues |
|
KD2083 |
TissueArray |
each |
EUR 474 |
|
Description: Kidney cancer tissue array with normal tissues, with stage and grade info, 69 cases/208 cores |
Kidney cancer tissue array with normal tissues |
|
KD721 |
TissueArray |
each |
EUR 168 |
|
Description: Kidney cancer tissue array with normal tissues, 24 cases/72 cores |
 Mouse Normal Tissue: Kidney Whole Cell Lysate |
|
MNK-100 |
Alpha Diagnostics |
1 mg |
EUR 628.8 |
Mouse Normal Tissue: Kidney Whole Cell Lysate |
|
MNK-50 |
Alpha Diagnostics |
50 ug |
EUR 196.8 |
 Normal Tissue: Kidney) Total RNA - Monkey (Rhesus) Normal Tissue: Kidney |
|
R1534142-50 |
Biochain |
50 ug |
EUR 191 |
 Kidney tumor tissue array with normal tissue as control |
|
KD961 |
TissueArray |
each |
EUR 258 |
|
Description: Kidney tumor tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores |
 Total Protein - Human Adult Normal Tissue: Kidney |
|
P1234142 |
Biochain |
1 mg |
EUR 188 |
 Kidney cancer tissue array with normal tissue as control |
|
KD962 |
TissueArray |
each |
EUR 258 |
|
Description: Kidney cancer tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores |
 Rat Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells |
|
9-80263 |
CHI Scientific |
1 x 100 ml |
Ask for price |
 Normal Tissue: Kidney) Total RNA - Monkey (Cynomolgus) Normal Tissue: Kidney |
|
R1534142-Cy |
Biochain |
50 ug |
EUR 191 |
 Membrane Protein - Human Adult Normal Tissue: Kidney |
|
P3234142 |
Biochain |
0.1 mg |
EUR 270 |
 Adjacent normal kidney tissue and cancer tissue array |
|
BN07011b |
TissueArray |
each |
EUR 203 |
|
Description: Adjacent normal kidney tissue and cancer tissue array, including pathology grade, TNM and clinical stage, 20 cases/54 cores, replacing BN07011 |
 tissue array with matched adjacent normal kidney tissue) Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue |
|
KD701a |
TissueArray |
each |
EUR 234 |
|
Description: Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 35 cases/70 cores, replacing KD701 |
 cDNA - Human Tumor Tissue: Kidney |
|
C1235142 |
Biochain |
40 reactions |
EUR 578 |
 Kidney cancer tissue array with matched adjacent normal tissue |
|
KD321a |
TissueArray |
each |
EUR 198 |
|
Description: Kidney cancer tissue array with matched adjacent normal tissue, including TNM, clinical stage and pathology ISUP grade, 16 cases/32 cores, replacing KD321 |
 Kidney cancer with matched adjacent normal kidney tissue array |
|
KD603 |
TissueArray |
each |
EUR 204 |
|
Description: Kidney cancer with matched adjacent normal kidney tissue array, containing metastatic clear cell carcinoma, including pathology grade, TNM and clinical stage (reference AJCC 8th version), 33 cases/61 cores |
 cDNA - Rat Normal Tissue: Skeletal Muscle |
|
C1434171 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Rat Normal Tissue: Small Intestine |
|
C1434226 |
Biochain |
40 reactions |
EUR 578 |
 Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue |
|
Kid-060Sur01 |
TissueArray |
each |
EUR 954 |
|
Description: Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue, including TNM, ISUP grade and survival data, 30 cases/60 cores |
 Normal Kidney Whole tissue lysate) Monkey (Cyno.) Normal Kidney Whole tissue lysate |
|
MCL-1190 |
Alpha Diagnostics |
1 mg |
EUR 628.8 |
 Whole tissue lysate) Human Adult Kidney (Normal) Whole tissue lysate |
|
HAL-1305 |
Alpha Diagnostics |
1 mg |
EUR 628.8 |
 Normal Kidney Whole tissue lysate) Monkey (Rhesus) Normal Kidney Whole tissue lysate |
|
MRL-1301 |
Alpha Diagnostics |
1 mg |
EUR 628.8 |
 Frozen Tissue Section - Human Adult Normal: Kidney |
|
T1234142 |
Biochain |
5 slides |
EUR 343 |
 Kidney Tumor Tissue Array - 12 cases of kidney cancer paired with adjacent normal tissues |
|
Z7020053 |
Biochain |
5 slides |
EUR 1125.6 |
|
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
 cDNA - Plant Normal Tissue: cDNA - Plant: Corn |
|
C1634330 |
Biochain |
40 reactions |
EUR 674 |
 cDNA - Plant Normal Tissue: cDNA - Plant: Rice |
|
C1634360 |
Biochain |
40 reactions |
EUR 674 |
 Paraffin Tissue Section - Human Adult Normal: Kidney |
|
T2234142 |
Biochain |
5 slides |
EUR 185 |
 Human Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells |
|
9-80075 |
CHI Scientific |
1 x 100 ml |
Ask for price |
 Mouse Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells |
|
9-80171 |
CHI Scientific |
1 x 100 ml |
Ask for price |
 cDNA - Plant Normal Tissue: cDNA - Plant: Wheat |
|
C1634390 |
Biochain |
40 reactions |
EUR 674 |
 cDNA - Plant Normal Tissue: cDNA - Plant: Orange |
|
C1634340 |
Biochain |
40 reactions |
EUR 674 |
 cDNA - Plant Normal Tissue: cDNA - Plant: Potato |
|
C1634350 |
Biochain |
40 reactions |
EUR 674 |
 cDNA - Dog Normal Tissue: Lung |
|
C1734152 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Dog Normal Tissue: Brain |
|
C1734035 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Dog Normal Tissue: Cecum |
|
C1734089 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Dog Normal Tissue: Heart |
|
C1734122 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Dog Normal Tissue: Liver |
|
C1734149 |
Biochain |
40 reactions |
EUR 385 |
 Multiple Kidney cancer tissue array with normal tissue control from autopsy |
|
KD483 |
TissueArray |
each |
EUR 168 |
|
Description: Multiple Kidney cancer tissue array with normal tissue control from autopsy, 48 cases/48 cores, with stage and grade data |
 cDNA - Dog Normal Tissue: Rectum |
|
C1734206 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Dog Normal Tissue: Testis |
|
C1734260 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Mouse Normal Tissue: Lung |
|
C1334152 |
Biochain |
40 reactions |
EUR 385 |
 Matched Pair - DNA - Human Primary Tumor and Normal Tissue: Kidney |
|
D8235142-PP-10 |
Biochain |
2x10 ug |
EUR 416 |
 cDNA - Human Diabetic Diseased Tissue: Kidney |
|
C1236142Dia |
Biochain |
40 reactions |
EUR 729 |
 cDNA - Dog Normal Tissue: Bladder |
|
C1734010 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Dog Normal Tissue: Trachea |
|
C1734160 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Dog Normal Tissue: Stomach |
|
C1734248 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Mouse Normal Tissue: Brain |
|
C1334035 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Mouse Normal Tissue: Colon |
|
C1334090 |
Biochain |
40 reactions |
EUR 578 |
 cDNA - Mouse Normal Tissue: Heart |
|
C1334122 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Mouse Normal Tissue: Liver |
|
C1334149 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Plant Normal Tissue: cDNA - Plant: Arabidopsis |
|
C1634310 |
Biochain |
40 reactions |
EUR 674 |
 Total RNA - Human Adult Normal Tissue 5 Donor Pool: Kidney |
|
R1234142-P |
Biochain |
50 ug |
EUR 370 |
 cDNA - Dog Normal Tissue: Pancreas |
|
C1734188 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Mouse Normal Tissue: Rectum |
|
C1334206 |
Biochain |
40 reactions |
EUR 578 |
 cDNA - Mouse Normal Tissue: Spleen |
|
C1334246 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Mouse Normal Tissue: Testis |
|
C1334260 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Plant Normal Tissue: cDNA - Plant: Soy bean |
|
C1634370 |
Biochain |
40 reactions |
EUR 674 |
 cDNA - Dog Normal Tissue: Esophagus |
|
C1734106 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Mouse Normal Tissue: Adipose |
|
C1334003 |
Biochain |
40 reactions |
EUR 578 |
 cDNA - Mouse Normal Tissue: Bladder |
|
C1334010 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Mouse Normal Tissue: Stomach |
|
C1334248 |
Biochain |
40 reactions |
EUR 385 |
 Rat Kidney Tissue Preparation Buffer 6: Normal Renal Firbroblasts |
|
9-80267 |
CHI Scientific |
1 x 100 ml |
Ask for price |
 (5 slides/pack)) Rat Normal Kidney tissue (single section per slide) (5 slides/pack) |
|
RaFPT007 |
TissueArray |
5 slides |
EUR 150 |
 cDNA - Mouse Normal Tissue: Placenta |
|
C1334200 |
Biochain |
40 reactions |
EUR 578 |
 Genomic DNA - Human Adult Normal Tissue: Kidney, from a single donor |
|
D1234142 |
Biochain |
100 ug |
EUR 286 |
 of kidney cancer and normal tissue) High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue |
|
KD208 |
TissueArray |
each |
EUR 546 |
|
Description: High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue |
 Matched Pair - Total RNA - Human Primary Tumor and Normal Tissue: Kidney |
|
R8235142-PP-10 |
Biochain |
2x10 ug |
EUR 463 |
 Mouse Kidney Tissue Preparation Buffer 3: Normal Podocytes |
|
9-80172 |
CHI Scientific |
1 x 100 ml |
Ask for price |
 cDNA - Human Adult Normal Tissue: Lung |
|
C1234152 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Human Adult Normal Tissue: Skin |
|
C1234218 |
Biochain |
40 reactions |
EUR 385 |
 Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue |
|
KD242 |
TissueArray |
each |
EUR 48 |
|
Description: Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue, 10 cases/24 cores |
 cDNA - Human Adult Normal Tissue: Brain |
|
C1234035 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Human Adult Normal Tissue: Colon |
|
C1234090 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Human Adult Normal Tissue: Heart |
|
C1234122 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Human Adult Normal Tissue: Liver |
|
C1234149 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Human Adult Normal Tissue: Ovary |
|
C1234183 |
Biochain |
40 reactions |
EUR 1049 |
 cDNA - Human Adult Normal Tissue: Theca |
|
C1234261-10 |
Biochain |
10 reactions |
EUR 215 |
 Normal Tissue: Lung) cDNA - Monkey (Rhesus) Normal Tissue: Lung |
|
C1534152 |
Biochain |
40 reactions |
EUR 385 |
 Normal Tissue: Skin) cDNA - Monkey (Rhesus) Normal Tissue: Skin |
|
C1534218 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Human Adult Normal Tissue: Breast |
|
C1234086 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Human Adult Normal Tissue: Rectum |
|
C1234206 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Human Adult Normal Tissue: Spleen |
|
C1234246 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Human Adult Normal Tissue: Testis |
|
C1234260 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Human Adult Normal Tissue: Throat |
|
C1234263-10 |
Biochain |
10 reactions |
EUR 215 |
 cDNA - Human Adult Normal Tissue: Thymus |
|
C1234264 |
Biochain |
40 reactions |
EUR 1049 |
 cDNA - Human Adult Normal Tissue: Tongue |
|
C1234267-10 |
Biochain |
10 reactions |
EUR 215 |
 cDNA - Human Adult Normal Tissue: Tonsil |
|
C1234268-10 |
Biochain |
10 reactions |
EUR 215 |
 cDNA - Human Adult Normal Tissue: Uterus |
|
C1234274 |
Biochain |
40 reactions |
EUR 385 |
 Kidney cancer with matched cancer adjacent normal tissue test array |
|
KD241 |
TissueArray |
each |
EUR 48 |
|
Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores |
Kidney cancer with matched cancer adjacent normal tissue test array |
|
KD241a |
TissueArray |
each |
EUR 66 |
|
Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores, replacing KD241 |
 Normal Tissue: Brain) cDNA - Monkey (Rhesus) Normal Tissue: Brain |
|
C1534035 |
Biochain |
40 reactions |
EUR 385 |
 Normal Tissue: Cecum) cDNA - Monkey (Rhesus) Normal Tissue: Cecum |
|
C1534089 |
Biochain |
40 reactions |
EUR 578 |
 Normal Tissue: Colon) cDNA - Monkey (Rhesus) Normal Tissue: Colon |
|
C1534090 |
Biochain |
40 reactions |
EUR 385 |
 Normal Tissue: Heart) cDNA - Monkey (Rhesus) Normal Tissue: Heart |
|
C1534122 |
Biochain |
40 reactions |
EUR 385 |
 Normal Tissue: Liver) cDNA - Monkey (Rhesus) Normal Tissue: Liver |
|
C1534149 |
Biochain |
40 reactions |
EUR 385 |
 Normal Tissue: Ovary) cDNA - Monkey (Rhesus) Normal Tissue: Ovary |
|
C1534183 |
Biochain |
40 reactions |
EUR 1049 |
 Normal Tissue: Penis) cDNA - Monkey (Rhesus) Normal Tissue: Penis |
|
C1534194 |
Biochain |
40 reactions |
EUR 578 |
 cDNA - Dog Normal Tissue: Skeletal Muscle |
|
C1734171 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Dog Normal Tissue: Small Intestine |
|
C1734226 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Human Adult Normal Tissue: Adipose |
|
C1234003-10 |
Biochain |
10 reactions |
EUR 215 |
 cDNA - Human Adult Normal Tissue: Adrenal |
|
C1234004 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Human Adult Normal Tissue: Bladder |
|
C1234010 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Human Adult Normal Tissue: Trachea |
|
C1234160-10 |
Biochain |
10 reactions |
EUR 215 |
 cDNA - Human Adult Normal Tissue: Stomach |
|
C1234248 |
Biochain |
40 reactions |
EUR 385 |
 cDNA - Human Adult Normal Tissue: Thyroid |
|
C1234265 |
Biochain |
40 reactions |
EUR 385 |
 Normal Tissue: Rectum) cDNA - Monkey (Rhesus) Normal Tissue: Rectum |
|
C1534206 |
Biochain |
40 reactions |
EUR 385 |
Tandem Mass Tag quantitative proteomics was utilized to disclose the temporal protein expression sample throughout osteogenic differentiation of hPDLSCs on days 0, 3, 7 and 14. Differentially expressed proteins (DEPs) have been clustered and purposeful annotated by Gene Ontology (GO) phrases. Pathway enrichment evaluation was carried out based mostly on the Kyoto Encyclopedia of Genes and Genomes database, adopted by the anticipated activation utilizing Ingenuity Pathway Evaluation software program. Interplay networks of redox-sensitive signalling pathways and oxidative phosphorylation (OXPHOS) have been performed and the hub protein SOD2 was validated with western blotting.
