The in vitro reconstruction of stromal tissue by long-term cultivation of corneal fibroblasts is a brilliant method for regenerative therapies of ocular floor ailments. Nevertheless, systematic investigations evaluating optimized cultivation protocols for the conclusion of a biomaterial are missing. This examine investigated the affect of dietary supplements to the tradition media of human corneal fibroblasts on the formation of a cell sheet consisting of cells and extracellular matrix. Among the many dietary supplements studied are vitamin C, fetal bovine serum, L-glutamine, elements of collagen reminiscent of L-proline, L-4-hydroxyproline and glycine, and TGF-β1, bFGF, IGF-2, PDGF-BB and insulin. After long-term cultivation, the proliferation, collagen and glycosaminoglycan content material and lightweight transmission of the cell sheets have been examined.
Biomechanical properties have been investigated by tensile exams and the ultrastructure was characterised by electron microscopy, small-angle X-ray scattering, antibody staining and ELISA. The synthesis of extracellular matrix was considerably elevated by cultivation with insulin or TGF-β1, every with vitamin C. The sheets exhibited a excessive transparency and appropriate materials properties. The manufacturing of a clear, scaffold-free, probably autologous, in vitro-generated assemble by culturing fibroblasts with extracellular matrix synthesis-stimulating dietary supplements represents a promising method for a biomaterial that can be utilized for ocular floor reconstruction in slowly progressing ailments.
Bioengineered 3D tunable neuronal constructs are a flexible platform for learning neuronal community features, providing quite a few benefits over current applied sciences and offering for the invention of latest organic insights. Practical neural networks could be evaluated utilizing calcium imaging and quantitatively described utilizing community science. This protocol contains directions for fabricating protein-based composite scaffolds, 3D in vitro tradition of embryonic mouse cortical neurons, virally induced expression of GCaMP6f, wide-field calcium imaging, and computational evaluation with open-source software program and customized MATLAB code. For full particulars on the use and execution of this protocol, please discuss with Dingle et al. (2020).
Mechanobiology in Tendon, Ligament, and Skeletal Muscle Tissue Engineering
Tendon, ligament, and skeletal muscle are highly-organized tissues that largely depend on a hierarchical collagenous matrix to face up to excessive tensile hundreds skilled in actions of every day life. This essential biomechanical position predisposes these tissues to damage, and present therapies fail to recapitulate the biomechanical perform of native tissue. This has prompted researchers to pursue engineering purposeful tissue replacements, or dysfunction/illness/improvement fashions, by emulating in vivo stimuli inside in vitro tissue engineering platforms; particularly mechanical stimulation, in addition to energetic contraction in skeletal muscle. Mechanical loading is essential for matrix manufacturing and group within the improvement, maturation, and upkeep of native tendon, ligament, and skeletal muscle, in addition to their interfaces.
Tissue engineers search to harness these mechanobiological advantages utilizing bioreactors to use each static and dynamic mechanical stimulation to tissue constructs, and induce energetic contraction in engineered skeletal muscle. The overwhelming majority of engineering approaches in these tissues are scaffold-based, offering interim construction and help to engineered constructs, and ample integrity to face up to mechanical loading. Alternatively, some latest research have employed developmentally-inspired scaffold-free strategies, counting on mobile self-assembly and matrix manufacturing to kind tissue constructs. Whether or not using a scaffold or not, incorporation of mechanobiological stimuli has been proven to enhance the composition, construction, and biomechanical perform of engineered tendon, ligament, and skeletal muscle. Collectively, these findings spotlight the significance of mechanobiology and recommend how it may be leveraged to engineer these tissues and their interfaces, and to create purposeful multi-tissue constructs.
Biomimetic Mineralization Promotes Viability and Differentiation of Human Mesenchymal Stem Cells in a Perfusion Bioreactor
In bone tissue engineering, the design of 3D programs able to recreating composition, structure and micromechanical atmosphere of the native extracellular matrix (ECM) continues to be a problem. Whereas perfusion bioreactors have been proposed as potential device to use biomechanical stimuli, its use has been restricted to a low variety of biomaterials. On this work, we suggest the tradition of human mesenchymal stem cells (hMSC) in biomimetic mineralized recombinant collagen scaffolds with a perfusion bioreactor to concurrently present biochemical and biophysical cues guiding stem cell destiny.
The scaffolds have been fabricated by mineralization of recombinant collagen within the presence of magnesium (RCP.MgAp). The natural matrix was homogeneously mineralized with apatite nanocrystals, comparable in composition to these present in bone. X-Ray microtomography photographs revealed isotropic porous construction with optimum porosity for cell ingrowth. In actual fact, an optimum cell repopulation via the whole scaffolds was obtained after 1 day of dynamic seeding within the bioreactor. Remarkably, RCP.MgAp scaffolds exhibited larger cell viability and a transparent development of up-regulation of osteogenic genes than management (non-mineralized) scaffolds. Outcomes reveal the potential of the mix of biomimetic mineralization of recombinant collagen in presence of magnesium and dynamic tradition of hMSC as a promising technique to carefully mimic bone ECM.
cDNA from Dog Normal Tissue: Kidney |
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| C1734142 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Kidney |
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| C1234142 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Kidney |
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| C1534142 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Kidney |
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| C1534142-Cy | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Kidney Tissue Lysate (Normal) |
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| 1706-02 | ProSci | 0.1 mg | EUR 260.7 |
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Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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Kidney Tissue Lysate (Normal) |
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| 1706-03 | ProSci | 0.1 mg | EUR 260.7 |
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Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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Kidney Tissue Lysate (Normal) |
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| 1706-04 | ProSci | 0.1 mg | EUR 260.7 |
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Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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Kidney Tissue Lysate (Normal) |
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| 1706-05 | ProSci | 0.1 mg | EUR 260.7 |
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Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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Kidney Tissue Slide (Normal) |
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| 10-401-10um | ProSci | 10 um | EUR 241.8 |
Kidney Tissue Slide (Normal) |
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| 10-401-4um | ProSci | 4 um | EUR 216.6 |
cDNA from Rat Normal Tissue: Adipose |
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| C1434003 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Rat Normal Tissue: Bladder |
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| C1434010 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Rat Normal Tissue: Brain |
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| C1434035 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Rat Normal Tissue: Colon |
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| C1434090 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Rat Normal Tissue: Heart |
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| C1434122 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Rat Normal Tissue: Liver |
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| C1434149 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Rat Normal Tissue: Lung |
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| C1434152 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Rat Normal Tissue: Placenta |
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| C1434200 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Rat Normal Tissue: Rectum |
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| C1434206 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Rat Normal Tissue: Spleen |
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| C1434246 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Rat Normal Tissue: Stomach |
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| C1434248 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Rat Normal Tissue: Testis |
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| C1434260 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Tumor Tissue: Kidney |
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| C1235142 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Diabetic Tissue: Kidney |
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| C1236142Dia | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Matching Pair - cDNA from Human Primary Tumor and Normal Tissue: Kidney |
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| C8235142-PP | Biochain | 10 reactions x2 | EUR 598.8 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Rat Normal Tissue: Skeletal Muscle |
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| C1434171 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Rat Normal Tissue: Small Intestine |
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| C1434226 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Genomic DNA from Rat Normal Tissue: Kidney |
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| D1434142 | Biochain | 100 ug | EUR 338.4 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Total RNA from Rat Normal Tissue: Kidney |
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| R1434142-50 | Biochain | 50 ug | EUR 182.4 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Mouse Normal Tissue: Adipose |
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| C1334003 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Mouse Normal Tissue: Bladder |
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| C1334010 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Mouse Normal Tissue: Brain |
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| C1334035 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Mouse Normal Tissue: Colon |
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| C1334090 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Mouse Normal Tissue: Heart |
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| C1334122 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Mouse Normal Tissue: Liver |
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| C1334149 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Mouse Normal Tissue: Lung |
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| C1334152 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Mouse Normal Tissue: Placenta |
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| C1334200 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Mouse Normal Tissue: Rectum |
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| C1334206 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Mouse Normal Tissue: Spleen |
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| C1334246 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Mouse Normal Tissue: Stomach |
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| C1334248 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Mouse Normal Tissue: Testis |
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| C1334260 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Dog Normal Tissue: Bladder |
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| C1734010 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Dog Normal Tissue: Brain |
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| C1734035 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Dog Normal Tissue: Cecum |
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| C1734089 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Dog Normal Tissue: Esophagus |
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| C1734106 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Dog Normal Tissue: Heart |
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| C1734122 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Dog Normal Tissue: Liver |
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| C1734149 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Dog Normal Tissue: Lung |
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| C1734152 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Dog Normal Tissue: Trachea |
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| C1734160 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Dog Normal Tissue: Pancreas |
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| C1734188 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Dog Normal Tissue: Rectum |
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| C1734206 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Dog Normal Tissue: Stomach |
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| C1734248 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Dog Normal Tissue: Testis |
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| C1734260 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Monkey (Cynomolgus) cDNA Normal Tissue: Liver |
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| MC34-149 | Alpha Diagnostics | 10 rxn | EUR 498 |
Monkey (Cynomolgus) cDNA Normal Tissue: Pancreas |
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| MC34-188 | Alpha Diagnostics | 10 rxn | EUR 498 |
Monkey (Cynomolgus) cDNA Normal Tissue: Spleen |
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| MC34-246 | Alpha Diagnostics | 10 rxn | EUR 498 |
cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis |
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| C1634310 | Biochain | 40 reactions | EUR 745.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Plant Normal Tissue: cDNA from Plant: Corn |
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| C1634330 | Biochain | 40 reactions | EUR 745.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Plant Normal Tissue: cDNA from Plant: Orange |
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| C1634340 | Biochain | 40 reactions | EUR 745.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Plant Normal Tissue: cDNA from Plant: Potato |
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| C1634350 | Biochain | 40 reactions | EUR 745.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Plant Normal Tissue: cDNA from Plant: Rice |
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| C1634360 | Biochain | 40 reactions | EUR 745.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Plant Normal Tissue: cDNA from Plant: Wheat |
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| C1634390 | Biochain | 40 reactions | EUR 745.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Rat Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells |
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| 9-80263 | CHI Scientific | 1 x 100 ml | Ask for price |
cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean |
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| C1634370 | Biochain | 40 reactions | EUR 745.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Adipose |
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| C1234003-10 | Biochain | 10 reactions | EUR 277.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Adrenal |
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| C1234004 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Bladder |
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| C1234010 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Brain |
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| C1234035 | Biochain | 40 reactions | EUR 451.2 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Breast |
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| C1234086 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Colon |
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| C1234090 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Esophagus |
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| C1234106 | Biochain | 40 reactions | EUR 451.2 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Heart |
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| C1234122 | Biochain | 40 reactions | EUR 451.2 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Liver |
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| C1234149 | Biochain | 40 reactions | EUR 451.2 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Lung |
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| C1234152 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Trachea |
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| C1234160-10 | Biochain | 10 reactions | EUR 277.2 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Ovary |
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| C1234183 | Biochain | 40 reactions | EUR 1126.8 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Pancreas |
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| C1234188 | Biochain | 40 reactions | EUR 451.2 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Placenta |
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| C1234200 | Biochain | 40 reactions | EUR 451.2 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Prostate |
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| C1234201 | Biochain | 40 reactions | EUR 451.2 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Rectum |
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| C1234206 | Biochain | 40 reactions | EUR 451.2 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Skin |
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| C1234218 | Biochain | 40 reactions | EUR 451.2 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Spleen |
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| C1234246 | Biochain | 40 reactions | EUR 451.2 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Stomach |
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| C1234248 | Biochain | 40 reactions | EUR 451.2 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Testis |
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| C1234260 | Biochain | 40 reactions | EUR 451.2 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Theca |
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| C1234261-10 | Biochain | 10 reactions | EUR 277.2 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Throat |
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| C1234263-10 | Biochain | 10 reactions | EUR 277.2 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Thymus |
|||
| C1234264 | Biochain | 40 reactions | EUR 1126.8 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
|||
cDNA from Human Adult Normal Tissue: Thyroid |
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| C1234265 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Tongue |
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| C1234267-10 | Biochain | 10 reactions | EUR 277.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Tonsil |
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| C1234268-10 | Biochain | 10 reactions | EUR 277.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Uterus |
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| C1234274 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Mouse Normal Tissue: Skeletal Muscle |
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| C1334171 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Mouse Normal Tissue: Small Intestine |
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| C1334226 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Adipose |
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| C1534003 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Adipose |
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| C1534003-Cy | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Adrenal |
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| C1534004 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Adrenal |
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| C1534004-Cy | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Appendix |
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| C1534006 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Appendix |
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| C1534006-Cy | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Bladder |
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| C1534010 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Bladder |
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| C1534010-Cy | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Brain |
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| C1534035 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Brain |
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| C1534035-Cy | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Brain:Cerebellum |
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| C1534039-Cy | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Cecum |
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| C1534089 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Cecum |
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| C1534089-Cy | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Colon |
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| C1534090 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Colon |
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| C1534090-Cy | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Epididymis |
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| C1534105 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Epididymis |
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| C1534105-Cy | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Esophagus |
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| C1534106 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Esophagus |
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| C1534106-Cy | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Gallbladder |
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| C1534118 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Gallbladder |
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| C1534118-Cy | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Heart |
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| C1534122 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Heart |
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| C1534122-Cy | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Liver |
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| C1534149 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Liver |
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| C1534149-Cy | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Lung |
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| C1534152 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Lung |
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| C1534152-Cy | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Trachea |
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| C1534160 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Trachea |
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| C1534160-Cy | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Diaphragm |
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| C1534169 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Diaphragm |
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| C1534169-Cy | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Ovary |
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| C1534183 | Biochain | 40 reactions | EUR 1126.8 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Ovary |
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| C1534183-Cy | Biochain | 40 reactions | EUR 1126.8 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Pancreas |
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| C1534188 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Pancreas |
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| C1534188-Cy | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Parathyroid |
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| C1534189 | Biochain | 40 reactions | EUR 1261.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Parathyroid |
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| C1534189-Cy | Biochain | 40 reactions | EUR 1261.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Parotid |
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| C1534190 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Parotid |
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| C1534190-Cy | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Penis |
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| C1534194 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Penis |
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| C1534194-Cy | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Placenta |
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| C1534200 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Placenta |
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| C1534200-Cy | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Prostate |
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| C1534201 | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Prostate |
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| C1534201-Cy | Biochain | 40 reactions | EUR 648 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Rectum |
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| C1534206 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Rectum |
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| C1534206-Cy | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Skin |
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| C1534218 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Skin |
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| C1534218-Cy | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Spleen |
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| C1534246 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Tandem Mass Tag quantitative proteomics was utilized to disclose the temporal protein expression sample throughout osteogenic differentiation of hPDLSCs on days 0, 3, 7 and 14. Differentially expressed proteins (DEPs) have been clustered and purposeful annotated by Gene Ontology (GO) phrases. Pathway enrichment evaluation was carried out based mostly on the Kyoto Encyclopedia of Genes and Genomes database, adopted by the anticipated activation utilizing Ingenuity Pathway Evaluation software program. Interplay networks of redox-sensitive signalling pathways and oxidative phosphorylation (OXPHOS) have been performed and the hub protein SOD2 was validated with western blotting.