Dr.-Thierry-Brune

Former Skin model Production Manager at Phenion (France)

2014-Now:
Staff Scientist, R&D Biopolymers / Absorbable Polymers – Medtronic (Lyon, France)
R&D team leader for new product development – Absorbable Polymers

  • Team management (Engineers & Lab. technicians)
  • Supervision of technical assays (Physico-chemistry & mechanical characterization)
  • Supervision of in vivo preclinical studies (small & large animal models)
  • Development of new testing methods
  • Scientific Support on Biologics (Quality assessment, Reviews, Complaints management…)
  • Clinicians training on biologics ; Scientific and technical watch

2011-2014:
Senior Scientist, R&D Biologics – Covidien (Lyon, France)
Support R&D team for new product development – Biologics & Antimicrobial Implants

  • Team management (Engineers & Lab. technicians)
  • Supervision of technical assays (Physico-chemistry & mechanical characterization)
  • Supervision of in vivo preclinical studies (small & large animal models, infected models)
  • Scientific support on biologics (Quality assessment, Reviews, Complaints management…)
  • Clinicians training on biologics ; Scientific and technical watch

2009-2011:
R&D Manager – TBF Tissue Engineering (Lyon, France)
Development of new products in orthopaedics tissue engineering (bone, cartilage, meniscus)

  • Management of a team made up of 4 people (project leaders & lab. technicians)
  • Multi project management (cell therapy products, processed tissues and medical devices)
  • Regulatory affairs and contribution to the continuous improvement of the quality system
  • In charge of drafting the scientific communications (lectures, articles, posters…)
  • Go-between with the key partners (clinicians, researchers, CRO…)
  • Scientific and technical watch

2006-2008:
Head of Production – Phenion GmbH & Co.KG, Subsidiary of Henkel KGaA (Düsseldorf, Germany)
Skin models development and production

  • Management of a team made up of 4 people (laboratory technicians and assistants)
  • Optimization of the processes for the production of skin models (epidermis and full-thickness)
  • Implementation of a new Quality Control Release (QCR) procedure; certification ISO9001:2000
  • Support for international customers (Germany, France, Belgium, Holland, Italy, Switzerland, USA)
  • Management of the logistic platform and shipments

2002-2006:
Project Leader – Natural Implant (Marseille, France)
Tissue engineering of the anterior cruciate ligament

  • Development of new ligament in vitro models based on autologous cells
  • Development of a new tubular reactor system for cell culture in perfusion
  • Management of several animal tests performed on different models (rabbit, dog, sheep, monkey)

2000:
Production & Research Assistant – Prevost Laboratory Concept (Rennes, France)

  • Cosmetics production and development of a new line of sunscreen products

Articles

  • Brune T, Borel A, Gilbert T, Franceschi JP, Badylak S, Sommer P. In vitro comparison of human fibroblasts from intact and ruptured ACL for use in tissue engineering. Eur Cell Mater. 2007 Dec 17;14:78-90.
  • Gault P, Black A, Romette JL, Fuente F, Schroeder K, Thillou F, Brune T, Berdal A, Wurtz T. Tissue-engineered ligament: implant constructs for tooth replacement. J Clin Periodontol. 2010 Jun 9. [Epub ahead of print].

Posters

  • Mewes KR, Brune T, Priessner A, Schröder KR. Elastic network in a full-thickness skin model. 5th European Meeting on Elastin. July 16-19, 2008. Alcala de Henares, Spain.
  • Brune T, Tan N, Duverneuil L, Gauduin A, Laganier L, Barnouin L. In vitro evaluation of an agarose-alginate-based hydrogel for chondrocytes implantation. 56th Annual Meeting of the Orthopaedic Research Society. March 6-9, 2010. New Orleans, Louisiana, USA.

Lectures

  • Brune T. Production of the Phenion® full-thickness skin model. Phenion 2nd Technical Workshop – « The Phenion® Skin Model Technologies and Their Applications ». May 27-28, 2008. Düsseldorf, Germany.
  • T. Brune, A. Schirlin, C. Martin, L. Barnouin. New process for the production of an acellular meniscus scaffold. 9th World Congress of the International Cartilage Repair Society. September 26-29, 2010. Sitges, Spain.
  • T. Brune, C. Martin, L. Barnouin. TKAMen: A new source for meniscus reconstruction. 9th World Congress of the International Cartilage Repair Society. September 26-29, 2010. Sitges, Spain.
  • T. Brune, N. Tan, L. Laganier, L. Barnouin. Agarose-alginate hydrogel as a scaffold for an allogeneic Cartipatch®. 9th World Congress of the International Cartilage Repair Society. September 26-29, 2010. Sitges, Spain.

Fields

Differentiation mechanisms :

  • Epidermal differentiation
  • Ligamentous differentiation
  • Chondrogenic differentiation
  • Mineralization and osteogenic differentiation

Extracellular matrix and cell adhesion:

  • ECM composition and collagenic turn-over
  • Elastic network
  • Cell adhesion mechanisms

Inflammation and associated mechanisms:

  • Inflammatory response
  • Mechanisms of immunity
  • Angiogenesis & wound healing
  • Epidermal irritation
  • Osteoarthritis
  • Biocompatibility, toxicity and mutagenicity

Models

  • 2D cell models
  • Skin – Reconstructed epidermis, « full-thickness » skin models
  • Cornea – Single epithelium, composite models epithelium and stroma
  • Cruciate ligament – Cells grown on a 3D structure made of SIS fibers
  • Periodontal  ligament – Cells grown on a titanium root covered with hydroxyapatite
  • Bone – Cells grown in hydroxyapatite scaffold or devitalized trabecular bone
  • 2008: Ph.D. in Biology – Claude Bernard University Lyon I (UCBL)
    Interdisciplinary Department Sciences and Health (EDISS)
    Tissue engineering of the anterior cruciate ligament
  • 2003: Postgraduate certificate in Biology – Méditerranée University Aix-Marseille II
    Eukaryotic biology: developmental, genetic and cellular aspects
  • 2002: Master’s degree in Biotechnology – Compiègne University of Technology (UTC)
    Department Invention and Development of Bioproducts (CIB)
  • 2001: University 6-months period of exchange – Paranà Catholic University (PUCPR, Brasil)
    Department of Biology
  • 1997: Scientific French high school graduation, focus on Mathematics, with honors